Multiplexing in Tissue
Pascal obtained his PhD from the University of Queensland, Australia, researching malignancy associated changes in the chromatin texture of cervical cell nuclei. His early career was spent developing automated cytology imaging systems at CSSIP in Australia in collaboration with Accumed International of Chicago. He subsequently took on the role of Director of Imaging at MonoGen where he developed a Digital Pathology solution for cervical cancer screening. Pascal joined Ventana Medical Systems four years ago and has held various roles in research and Digital Pathology, working on fluorescence multiplexing imaging systems and driving several brightfield IHC algorithm CE-IVD and 510(k) clearances. Pascal is currently the Project Leader for Multiplex and Molecular Assays, based in Tucson.
Multiplexing genomic and proteomic targets in Formalin-Fixed, Paraffinembedded (FFPE) tissue is widely regarded as a key enabling technology in the future of personalized medicine and companion diagnostics. Several approaches have been suggested; from serial section staining and analysis, to single slide multi-labeling with Quantum Dots. These techniques have a common theme in the form of requiring extensive image analysis and statistical pattern recognition, disciplines wholly contained within the field of Digital Pathology. Of upmost importance is the translation of these systems to the clinical setting, where workflow is paramount to ensure that the resulting assay is simple to use, robust and highly repeatable. We shall present an overview of multiplexing in tissue with an emphasis on the resulting in-vitro diagnostic test.
At the end of this program, participants should be able to be conversant in multiplexing techniques in tissue, the reasons why this is a complelling area of development and current progress in the field.
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